| Zhenpeng Zhao,Yuanyong Feng,Wei Li,Shengbiao Wan,Tao Jiang,Wei Shang. Study of the Dextran-DTPA-Gd at rabbit popliteal fossa lymph node metastasis from thigh transplanted tumor with interstitial magnetic resonance lymphography. Oncol Transl Med, 2014, 13: 240-244. |
| Dextran-DTPA-Gd兔大腿移植瘤腘窝淋巴转移间质磁共振淋巴造影的研究 |
| Study of the Dextran-DTPA-Gd at rabbit popliteal fossa lymph node metastasis from thigh transplanted tumor with interstitial magnetic resonance lymphography |
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| Author Name | Affiliation | | Zhenpeng Zhao | Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University,
Qingdao 266003, China | | Yuanyong Feng | Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University,
Qingdao 266004, China | | Wei Li | Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Hospital of Medical College, Qingdao
University, Qingdao 266003, | | Shengbiao Wan | Key Laboratory of Marine Drugs, Ministry of Education
School of Pharmacy, Ocean University of China, Qingdao
266003, China | | Tao Jiang | Key Laboratory of Marine Drugs, Ministry of Education
School of Pharmacy, Ocean University of China, Qingdao
266003, China | | Wei Shang | Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University,
Qingdao 266003, China |
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| 中文摘要: |
| 目的：Dextran-DTPA-Gd行兔大腿VX2移植瘤腘窝淋巴转移间质核磁共振淋巴造影实验，探讨其靶向强化转移淋巴结及淋巴管的显影情况。方法：选取健康新西兰大白兔12只，将兔VX2肿瘤移植至兔子右侧后肢股四头肌，左侧作为对比侧，其中8只大白兔1个月后发生同侧腘窝淋巴结转移，采用3.0 GE 核磁共振检查。将发生腘窝淋巴转移的8只大白兔每只双侧后肢趾蹼间隙注射Dextran-Gd-DTPA靶向淋巴造影剂各0.4ml（3.96×10-3mol/L）。行MR增强扫描，间隔均分别为10、15、20、25、30、35、40、45、50、55、60min、2h、4h、24h。计算增强前后双侧腘窝淋巴结的信号强度（E%），绘制腘窝淋巴结信号强度-时间曲线，观察癌转移淋巴结及淋巴管显影情况，比较两侧腘窝淋巴结显影的不同。结果：双侧后肢经趾蹼间隙Dextran-Gd-DTPA注射10min后，后肢淋巴管及腘窝淋巴结显影明显、均匀强化，血管无显影。于35min左右达到峰值，E%为315%，注射4h后E%为205%，24h后扩清。腘窝淋巴结在不同时间的信号强化率进行统计学分析，差别有统计学意义（P﹤0.05）。肿瘤侧腘窝淋巴结因肿瘤侵染，表现为周围毛糙状不规则状，淋巴管迂曲增粗，淋巴链不完整。结论：Dextran-DTPA-Gd对淋巴组织显影具有特异性，能快速靶向显示引流区域淋巴管走形，正常及发生癌转移淋巴结的形态。 |
| 英文摘要: |
| Objective: The aim of the study was to investigate the developing situation of the interstitial magnetic resonance (MR) lymphoid contrast agent Dextran-DTPA-Gd through the rabbit popliteal fossa lymph node metastasis from thigh VX2 transplanted tumor injection to show targeting enhanced metastatic lymph nodes and lymphatics. Methods: VX2 tumor was transplanted to the right hind limb quadriceps of 12 healthy New Zealand rabbits and the left side as a contrast. Eight rabbits had homonymy popliteal lymph node metastasis after 1 month through 3.0 GE MRI and they were later injected with lymphatic targeting contrast agent Dextran-DTPA-Gd 0.4 mL (3.96 × 10–3 mol/L) through bilateral hindlimb toe web respectively. Enhanced MR images were obtained with interval 10 min, 15 min, 20 min, 25 min, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min, 60 min, 2 h, 4 h, and 24 h. The signal intensities before and after enhancing were measured to calculate the enhancing rates (E%) of popliteal lymph node and the popliteal lymph node signal intensity-time curves were drawn to observe the development of cancer metastasis lymph nodes and lymphatics and to compare the differences of interval sides. Results: Ten minutes after injected into the rabbit’s bilateral hindlimb toe web, we could see hind lymphatic and popliteal lymph nodes were strengthened significantly and evenly without blood vessels developing. The signal reached a peak after 35 min with E% to 315%, which decreased to 205% after 4 h and would be undifferentiated with the surrounding tissues after 24 h. Statistical analysis was made to popliteal lymph node enhancement rate. It was considered statistically significant as long as P < 0.05. The tumor-side popliteal lymph node manifested as coarse and irregular shape, lymphatic vessels tortuous dilated and lymphatic chain incomplete as a result of tumor infection. Conclusion: Dextran-DTPA-Gd is specific to lymphoid tissue development. It can targeting display regional lymphatic drainage concretion and the morphology of normal and cancer cells metastasis lymph nodes rapidly. |
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