| Yaqiong Ni,Qinjiang Liu,Shihong Ma,Ruihui Chen. Inhibition effects of parathyroid hormone on human medullary thyroid carcinoma cells. Oncol Transl Med, 2014, 13: 224-228. |
| 甲状旁腺素对人甲状腺髓样癌细胞增值抑制的实验研究 |
| Inhibition effects of parathyroid hormone on human medullary thyroid carcinoma cells |
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| DOI： |
| 中文关键词: 甲状旁腺素；甲状腺髓样癌细胞株；甲状旁腺素受体单抗 |
| 英文关键词: parathyroid hormone (PTH); medullary thyroid carcinoma (MTC) cell line; parathyroid hormone receptor monoclonal antibody |
| 基金项目:兰州市科技计划项目（2013-3-38） |
| Author Name | Affiliation | | Yaqiong Ni | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730050, China | | Qinjiang Liu | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730051, China | | Shihong Ma | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730052, China | | Ruihui Chen | Department of Head and Neck Surgery, The Cancer Hospital of Gansu Province, Lanzhou 730053, China |
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| 中文摘要: |
| 目的 探讨甲状旁腺素和甲状旁腺素受体单抗对人甲状腺髓样癌细胞株体外生长及增殖的影响。方法 体外培养甲状腺髓样癌细胞株，经甲状旁腺素和甲状旁腺素受体单抗干预处理后，在倒置相差显微镜下观察细胞的生长状况，MTT法检测细胞生长抑制率。结果 倒置相差显微镜下细胞变化明显，甲状旁腺素和甲状旁腺素受体单抗均能有效地抑制甲状腺髓样癌细胞增殖，呈时间和剂量依赖。当甲状旁腺素浓度为2.0μmol/L 、甲状旁腺素受体单抗浓度为1.0μmol/L时，显著抑制细胞生长（P＜0.05）。结论 甲状旁腺素和甲状旁腺素受体单抗分别能抑制甲状腺髓样癌细胞的增殖，明显降低其增殖指数。 |
| 英文摘要: |
| Objective: The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma cell lines. Methods: The medullary thyroid carcinoma cell line was cultured in vitro, with parathyroid hormone and parathyroid hormone receptor monoclonal antibody treatment intervention, the growth of the cells was observed under an inverted contrast microscope, the MTT assay was used to detect the cell growth inhibition rate. Results: Under the inverted contrast microscope, the cells changed significantly, the parathyroid hormone and parathyroid hormone receptor monoclonal antibodies can effectively inhibit the proliferation of medullary thyroid cancer cells in a time and dose dependent. When parathyroid hormone concentration reached a concentration of 2.0 μmol/L, the parathyroid hormone receptor monoclonal antibody reached a concentration of 1.0 μmol/L, the cell growth was most significantly inhibited (P < 0.05). Conclusion: Parathyroid hormone and parathyroid hormone receptor monoclonal antibody were able to inhibit the proliferation of medullary thyroid carcinoma cells and significantly reduce the proliferation index. |
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