| Rumana Jafarey,Jing Yang. Morphology and survival of cryopreserved-thawed ovarian tissues after heterotopic autotransplantation. Oncol Transl Med, 2014, 13: 110-114. |
| 冷冻-解冻卵巢组织异位自体移植后的形态学及存活状态的研究 |
| Morphology and survival of cryopreserved-thawed ovarian tissues after heterotopic autotransplantation |
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| DOI： |
| 中文关键词: 卵巢组织，形态学，异位移植，解冻 |
| 英文关键词: ovarian tissue; morphology; heterotopic transplantation; thawing |
| 基金项目: |
| Author Name | Affiliation | | Rumana Jafarey | Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan 430060, China | | Jing Yang | Reproductive Medical Centre, Renmin Hospital of Wuhan University, Wuhan 430061, China |
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| 中文摘要: |
| 目的 观察并比较解冻后的卵巢组织经异位移植后的存活情况及形态学改变。方法 将20只5-6周龄的SPF级雌鼠随机分成两组，每组20只小鼠。对照组（组1：新鲜卵巢组）小鼠新鲜分离的卵巢用福尔马林液固定保存，而实验组（组2：玻璃花卵巢组）小鼠的卵巢离体后迅速进行玻璃化，然后做成薄薄的切片置入液氮中冷冻保存21天，之后迅速解冻并移植入小鼠背部肌肉内。卵巢组织移植2周和4周后，分别处死各小鼠，收集移植的卵巢组织，用4%的甲醛溶液固定，经HE染色后在光共聚焦显微镜下观察。结果 肉眼观察卵巢组织，除了尺寸有所缩小和颜色有所改变外形态上大体没有什么变化。在显微镜下观察发现卵巢保持正常的皮质和髓质结构，而且皮质内可见完整的始基卵泡和处于不同发育阶段的卵泡，但是移植后的卵巢组织（包括移植2周和4周）内很少看到正常的卵母细胞，显著少于新鲜组卵巢组织内的正常卵母细胞。结论 玻璃化冷冻后移植组的卵巢组织均显示较好的存活力，而且移植不同时间段（2周和4周）后的卵巢组织内处于不同发育阶段的卵泡的比例没有明显差异。该结果提示卵巢组织经冷冻后异位移植入小鼠模型背部肌肉内可以存活，而且保持其正常形态及功能。 |
| 英文摘要: |
| Objective: The aim of our study was to observe the survival and morphological changes of thawed ovarian tissues after heterotopic transplantation. Methods: Twenty SPF-SD female rats (5–6 weeks old) were equally randomized into the control group and experimental group. In control group, the freshly isolated ovaries were fixed in formalin. In experimental group, the freshly isolated ovaries were vitrified immediately and cut into thin slices. After stored in liquid nitrogen for 21 days, the tissues of experimental group were rapidly thawed and transplanted into back muscles of rats for 2 or 4 weeks, respectively. After that, all rats in experimental group were sacrificed and the ovarian tissues were collected and fixed in 4% formaldehyde solution. Then the ovarian tissues were stained with HE and observed under the light confocal microscope. Results: With the naked eyes, there was no specific alteration except the size reduction with color changing. Under microscopy, we found normal cortex and medulla in the ovary, and the primordial follicles and follicles in various stages were observed in the cortex. The normal oocytes in ovarian tissues of experimental group were significant decreased than in the control group. Conclusion: The ovarian tissues survive well in experimental group and there is no significant difference in the proportion of follicles between different times (2 and 4 weeks) after grafting. Our results suggest that thawed ovarian tissues could survive after heterotopic transplantation into back muscles of rat models and maintain their morphology and function. |
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