| Yanlin Cai,Zhongdong Chen,Aiqiong Tang,Bin Jiang,Zhaohua Fan,Jun Bai. Effects of the anti-tumor composition of the acetoacetate extract of vitex negundo seed on the growth of human cervical carcinoma xenografts in nude mice. Oncol Transl Med, 2014, 13: 105-109. |
| 黄荆子抗肿瘤活性成分对宫颈癌裸鼠移植瘤生长的影响 |
| Effects of the anti-tumor composition of the acetoacetate extract of vitex negundo seed on the growth of human cervical carcinoma xenografts in nude mice |
| |
| DOI： |
| 中文关键词: 宫颈癌；EVn-50；移植瘤；凋亡 |
| 英文关键词: cervical cancer; EVn-50; xenografts; apoptosis |
| 基金项目:湖南省衡阳市科技计划项目（2011KJ36） |
| Author Name | Affiliation | | Yanlin Cai | Deparetment of Gynecolgy, The First Affiliated Hospital of University of South China, Hengyang 421001, China | | Zhongdong Chen | Deparetment of Gynecolgy, The First Affiliated Hospital of University of South China, Hengyang 421002, China | | Aiqiong Tang | Deparetment of Gynecolgy, Hunan Provincial Maternal and Child Health Hospital, Changsha 410008, China | | Bin Jiang | Department of Plastic Surgery, The Second Affiliated Hospital of University of South China, Hengyang 421001,China | | Zhaohua Fan | Deparetment of Gynecolgy, The First Affiliated Hospital of University of South China, Hengyang 421001, China | | Jun Bai | Deparetment of Obstetrics and Gynecolgy, Hangzhou Red Cross Hospital, Hangzhou 310003, China |
|
| Hits: 9974 |
| Download times: 11951 |
| 中文摘要: |
| 目的 研究黄荆子抗肿瘤活性成分对人宫颈癌HeLa细胞裸鼠移植瘤生长的影响。方法 建立人宫颈癌HeLa细胞裸鼠皮下移植瘤模型，随机分成7组，每组5只：NS组、TAX组、EVn-50组、comp-6组、comp-7组、comp-8组和comp-10组。观察和比较各组裸鼠移植瘤的体积和重量，裸鼠体重的变化，裸鼠血清乳酸脱氢酶、谷丙转氨酶、肌酐值和外周血白细胞计数的变化；FCM测定瘤组织细胞凋亡率；Western blot分析瘤组织细胞内P53和Bcl-2的蛋白表达。结果EVn-50及其分级分离提取物可显著抑制人宫颈癌HeLa细胞裸鼠移植瘤的体积和重量的增加，呈时间依赖性；而对裸鼠的体重、谷丙转氨酶、乳酸脱氢酶、肌酐值和外周血白细胞计数均无明显的影响。EVn-50及其分级分离提取物作用16天后，裸鼠移植瘤细胞呈现明显的凋亡，同时P53蛋白表达上调，而Bcl-2蛋白表达下调。结论EVn-50及其分级分离提取物可能通过上调P53蛋白，下调Bcl-2蛋白促使瘤细胞凋亡，而抑制人宫颈癌裸鼠移植瘤生长。 |
| 英文摘要: |
| Objective: The aim of our study was to investigate the effects of the anti-tumor composition of the acetoacetate extract of Vitex Negundo Seed (EVn-50) on the growth of human cervical carcinoma HeLa cells xenografts in nude mice and its possible molecular mechanism. Methods: Models of human cervical cancer HeLa cells xenografts transplanted subcutaneously in nude mice were established and randomly divided into 7 groups (each group including 5 nude mice): saline group, Taxol group, EVn-50 group, comp-6 group, comp-7 group, comp-8 group and comp-10 group. The volume and weight of Xenograts were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of LDH, ALT, Cr and WBC counts were examined and compared. The apoptotic rate of human cervical carcinoma HeLa cells xenografts was analyzed by FCM. The expressions of P53 and Bcl-2 proteins of HeLa cells xenografts were determined by Western blotting. Results: EVn-50 and its fractionated extracts could significantly suppress the increasing volume and weight of human cervical carcinoma HeLa cells xenografts in nude mice models in time-dependent manner, yet had no significant effect on the weight of nude mice, the serum levels of LDH, ALT, Cr and WBC were counted. When the xenografts were treated with EVn-50 and its fractionated extracts for 16 days, the apoptotic rate of xenografts cells were significantly increased, and the expression of P53 protein was up-regulated and protein level of Bcl-2 was decreased. Conclusion: EVn-50 and its fractionated extracts could suppress the growth of human cervical carcinoma HeLa cells xenografts in nude mice, which may be related to its promotion on xenografts cells apoptosis through down-regulation of Bcl-2 expression and activation of P53 expression. |
|
View Full Text
Download reader HTML全文 |
| Close |
|
|
|