| Shu Huang,Ganxin Wang. Xiaoaiping injection affects the invasion and metastasis of hepatocellular carcinoma by controlling AFP expression. Oncol Transl Med, 2023, 9: 35-42. |
| Xiaoaiping injection affects the invasion and metastasis of hepatocellular carcinoma by controlling AFP expression |
| Received:November 11, 2022 Revised:March 06, 2023 |
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| KeyWord:Xiaoaiping injection; Alpha-fetoprotein (AFP); hepatocellular carcinoma (HCC); invasion, metastasis |
| Author Name | Affiliation | E-mail | | Shu Huang | Department of Hepatology of Integrated Traditional Chinese and Western Medicine, The Third People''''s Hospital of Hubei Province affiliated with Jianghan University | huangshu__2020@163.com | | Ganxin Wang | Department of Hepatology of Integrated Traditional Chinese and Western Medicine, The Third People''''s Hospital of Hubei Province affiliated with Jianghan University | Medicine_wgx@163.com |
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| Abstract: |
| Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancer
drug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibit
the growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker and
is closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression and
XAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, we
aimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..
Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the different
invasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowed
comparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.
AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasis
associated with AFP and XAP treatment.
Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in the
control group (P < 0.05). Additionally, compared to the control group, the expression of AFP significantly
decreased after XAP treatment (P < 0.05). The ability of Hep3B cells to invade and metastasize was
promoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAP
injection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases
(MMPs).
Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expression
of AFP; additionally, this inhibition of AFP is achieved by affecting MMPs. |
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