| Fajiang Mao,maofajiang,Xingli Wu,wuxingli,yuanchenyue,Chenyue Yuan,huanghaiyan,Haiyan Huang,Yanyan Qiu,qiuyanyan,renjianlin,Jianlin Ren,yinpeihao. Allosteric probe-modified liposome loading bufalin-fluorouracil complex for targeted colorectal cancer therapy. Oncol Transl Med, 2022, 8: 239-246. |
| Allosteric probe-modified liposome loading bufalin-fluorouracil complex for targeted colorectal cancer therapy |
| Received:June 30, 2022 Revised:October 24, 2022 |
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| KeyWord:bufalin; fluorouracil; colorectal cancer; imaging; therapy |
| Author Name | Affiliation | Postcode | | Fajiang Mao | Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine | 200071 | | maofajiang | Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese | 200071 | | Xingli Wu | Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine | 200071 | | wuxingli | Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine | | | yuanchenyue | Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine | | | Chenyue Yuan | Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine | 200071 | | huanghaiyan | Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine | | | Haiyan Huang | Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine | 200071 | | Yanyan Qiu | Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine | 200071 | | qiuyanyan | Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine | | | renjianlin | Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine | | | Jianlin Ren | Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine | 200071 | | yinpeihao | Interventional Cancer Institute of Chinese Integrative Medicine，Putuo Hospital, Shanghai University of Traditional Chinese Medicine | 200070 |
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| Abstract: |
| Objective Bufalin, the main active anti-tumor monomer of toad venom, is crucial in cancer treatment.
However, intrinsic issues, such as poor solubility and systematic toxicity, have considerably mitigated
its anticancer functions and caused unwanted side effects. It is essential to develop innovative targeting
systems to precisely and efficiently deliver anticancer drugs to achieve satisfying therapeutic efficiency.
Methods This work established a novel and more efficient system for simultaneously detecting and
killing colorectal cancer cells. The proposed method designed two allosteric probes, a report probe and
a recognize probe. The method exhibited high sensitivity towards cell detection via the recognizing probe
identifying target cancer cells and the report probe’s signal report. Combining bufalin and fluorouracil
endowed better tumor cell inhibition.
Results We observed significantly enhanced fluorescence dots surrounding the HCT-116 cell membranes.
No fluorescence increments in the other three cells were identified, indicating that the established liposome
complex could specifically bind with target cells. In addition, the best ratio of bufalin to fluorouracil was
0.15 and 0.5, respectively. This improved the anti-tumor effects and achieved more than 60% tumor cell
inhibition.
Conclusion This method will provide new opportunities for intracellular biomolecule detection and
targeted cancer cell therapy. |
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