| Mmengting Dong,Jiayu Sheng,Chunyang Li,Patiguli·JIAPAER,Xiaofei Li,Minjia Yuan,Xiaohong Xue,Ke Jiang. CD14 macrophage and IL-10 levels in the. Oncol Transl Med, 2022, 8: 165-172. |
| CD14 macrophage and IL-10 levels in the |
| Received:November 27, 2021 Revised:August 09, 2022 |
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| KeyWord:breast cancer; macrophage; IL-10; peripheral blood; diagnostic value |
| Author Name | Affiliation | E-mail | | Mmengting Dong | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | nancyd33@163.com | | Jiayu Sheng | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Chunyang Li | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Patiguli·JIAPAER | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Xiaofei Li | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Minjia Yuan | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Xiaohong Xue | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | | | Ke Jiang | Department of Breast Diseases, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China | surgeonjk@163.com |
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| Abstract: |
| Objective To explore the correlation between macrophages and interleukin-10 (IL-10 in the peripheral
blood of breast cancer (BC) patients and the diagnostic value of joint detection.
Methods BC patients (n = 50) and healthy controls (n = 40) were prospectively recruited. The percentage
of circulating cluster of differentiation 14 (CD 14) macrophage cells was analyzed by flow cytometry, and
an enzyme-linked immunosorbent assay (ELISA) was used to detect IL-10 expression levels. Receiver
operating characteristic (ROC) curves were used to verify the diagnostic value of the models based on the
expression of CD14 macrophage cell populations and IL-10. In addition, the association between model
expression and clinicopathological characteristics was investigated. Another 30 patients with BC and 30
with benign breast disease were selected to validate the IL-10 and CD14 macrophage joint detection model
using the same method.
Results CD14 macrophage and IL-10 expression levels in BC patients were higher than those in healthy
controls (P < 0.05). The ROC curve showed that the area under the curve (AUC) of CD14+ macrophages
combined with IL-10 was 0.830, the sensitivity was 72.0%, and the specificity was 87.5%. Its diagnostic
efficiency was better than all other single and joint detections. Correlation analysis of clinicopathological
features showed that IL-10 and CD14+ macrophage joint detection was significantly correlated with tumor
size, tumor-node-metastasis (TNM) stage, and lymph node, estrogen receptor (ER), and Ki-67 expression
(P < 0.05). The validation analysis results were consistent with the test results.
Conclusion Peripheral blood macrophages can be an independent diagnostic marker for BC. Joint
detection of CD14- macrophages and IL-10 suggests poor prognosis, which has unlimited potential to guide
BC development and provides a new theory for studying tumor-associated macrophages in BC. |
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