| Hua Yang,Yuxue Wei,Gangli Liu. Identification of microRNA-21 as a valuable diagnostic marker of oral squamous cell carcinoma and potential target. Oncol Transl Med, 2021, 7: 195-202. |
| Identification of microRNA-21 as a valuable diagnostic marker of oral squamous cell carcinoma and potential target |
| Received:May 11, 2021 Revised:October 27, 2021 |
| View Full Text View/Add Comment Download reader |
| KeyWord:miR-21; oral squamous cell carcinoma (OSCC); diagnostic meta-analysis; target gene prediction |
| Author Name | Affiliation | Postcode | | Hua Yang | Department of Stomatology, People''s Hospital of Lanling County | 277700 | | Yuxue Wei | Department of Stomatology, People''s Hospital of Lanling County | | | Gangli Liu | Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, School of Stomatology, Qilu Medical College, Shandong University | 250012 |
|
| Hits: 3239 |
| Download times: 3966 |
| Abstract: |
| Objective The aim of the study was to summarize the diagnostic value of miR-21 as a biomarker in oral
squamous cell carcinoma (OSCC) using a review of the literature and data from the cancer genome atlas
(TCGA) database.
Methods Data from TCGA database was sorted and analyzed by bioinformatics to determine the
expression level of miR-21 in OSCC. Further, we searched for relevant articles in Embase, PubMed/
Medline, Scopus, and Web of Science published before March 2021, extracted the data, and conducted
quality assessment. The bivariate meta-analysis model with Stata 16.0 was used to analyze the diagnostic
value of miR-21 for OSCC.
Results A total of 304 related articles were identified, and seven were selected for meta-analysis. The
diagnostic results after analysis were as follows: sensitivity 0.76 [95% confidence interval (CI), 0.57–0.88];
specificity 0.77 (95% CI, 0.58–0.89); positive likelihood ratio 3.34 (95% CI, 1.58–7.08); negative likelihood
ratio 0.31 (95% CI, 0.15–0.63); diagnostic odds ratio 10.75 (95% CI, 2.85–40.51); and area under the curve
0.83 (95% CI, 0.80–0.86). The Deeks’ funnel chart showed that there was no potential bias (P = 0.54).
Prediction analysis of the potential target genes of miR-21 was performed via the biological website, and
DAVID was used to cross target genes for gene ontology (GO) annotation function analysis.
Conclusion The results showed that miR-21-3p and miR-21-5p were significantly more highly expressed
in OSCC tissues than in normal tissues (P < 0.05), and the results of the meta-analysis indicated that they
could be used as potential biomarkers in the diagnosis of OSCC. In addition, 58 potential target genes of
miR-21 were significantly enriched in 28 GO annotation functional pathways, which provided a biological
basis for further clinical diagnostic value research. |
| Close |
|
|
|