| Tingting Hao,Chaoqi Wang,Yingjie Song,Wanyan Wu,Xuetao Li,Tao Fan. Relationship between miR-7-5p expression and 125I seed implantation efficacy in pancreatic cancer and functional analysis of target genes. Oncol Transl Med, 2021, 7: 177-182. |
| Relationship between miR-7-5p expression and 125I seed implantation efficacy in pancreatic cancer and functional analysis of target genes |
| Received:May 07, 2021 Revised:August 23, 2021 |
| View Full Text View/Add Comment Download reader |
| KeyWord:miR-7-5p; pancreatic cancer; 125I; radioactive seed implantation |
| Author Name | Affiliation | E-mail | | Tingting Hao | The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | 304150601@qq.com | | Chaoqi Wang | Department of Urinary Surgery, Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao 028007, China | | | Yingjie Song | General Surgery, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | | | Wanyan Wu | Department of Oncology, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | | | Xuetao Li | Department of Oncology, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | | | Tao Fan | Department of Oncology, The People''s Hospital of China Three Gorges University, The First People''s Hospital of Yichang, Yichang 443000, China | 1601340054@qq.com |
|
| Hits: 3366 |
| Download times: 3563 |
| Abstract: |
| Objective The aim of this study was to investigate the relationship between miR-7-5p expression and
intertissue-125I irradiation sensitivity in pancreatic cancer tissues and to analyze the function of target genes.
Methods Thirty-seven patients with unresectable pancreatic ductal adenocarcinoma (PDAC) treated with
radioactive 125I seed implantation were enrolled. RT-PCR was used to detect the expression level of miR-7-
5p in cancer tissues and analyze the relationship between miR-7-5p expression and 125I radiation sensitivity.
Bioinformatic software and online tools were used to predict the miR-7-5p target genes and analyze their
functional annotation and pathway enrichment.
Results Radioactive 125I seed implantation was followed up for 2 months. The objective response rate of
the miR-7-5p high expression group was 65.0% (13/20), whereas the objective response rate of the miR-
7-5p low expression group was 5.88% (1/17), and the difference between the two groups was statistically
significant (χ2 = 13.654, P < 0.001). A total of 187 target genes were predicted using three databases.
GO functional annotation showed that target genes were mainly involved in cellular response to insulin
stimulus, regulation of gene expression by genetic imprinting, cytosol, peptidyl-serine phosphorylation,
bHLH transcription factor binding, cargo loading into vesicles, cellular response to epinephrine stimulus,
and nucleoplasm. KEGG pathway enrichment analysis showed that target genes were mainly involved
in the ErbB signaling pathway, HIF-1 signaling pathway, axon guidance, longevity regulatory pathway,
endocrine resistance, glioma, choline metabolism in cancer, and EGFR tyrosine kinase inhibitor drug
resistance. Molecular complex detection analysis by Cytoscape revealed that PIGH, RAF1, EGFR, NXT2,
PIK3CD, PIK3R3, ERBB4, TRMT13, and C5orf22 were the key modules of miR-7-5p target gene clustering.
Conclusion The expression of miR-7-5p in pancreatic cancer tissues positively correlated with the
radiosensitivity of 125I seeds. Via targeted gene regulation, miR-7-5p acts on the network of multiple
signaling pathways in PDAC and participates in its occurrence and development. Thus, miR-7-5p may
become a predictive index of 125I seed implantation therapy sensitivity in PDAC patients. |
| Close |
|
|
|