| Xia Chen,Xiaoming Zhang,Xiangji Lu,Meng Ren,Rina Su,Weishi Gao,Yanwei Gao. Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer*. Oncol Transl Med, 2021, 7: 165-171. |
| Enhancing the treatment effects of tumor cell purified autogenous heat shock protein 70-peptide complexes on HER-3-overexpressing breast cancer* |
| Received:February 24, 2021 Revised:July 29, 2021 |
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| KeyWord:heat shock protein 70 peptide complexes (HSP70-PCs); HER-3 protein; recombinant protein; dendritic cells (DCs); cellular immunotherapy |
| Author Name | Affiliation | Postcode | | Xia Chen | Inner Mongolia Red Cross Blood Center, Hohhot, Inner Mongolia 010010, China | 010010 | | Xiaoming Zhang | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China | | | Xiangji Lu | Inner Mongolia Armed Police Hospital, Hohhot, Inner Mongolia 010010, China | | | Meng Ren | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China | | | Rina Su | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China | | | Weishi Gao | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China * | | | Yanwei Gao | Inner Mongolia People’s Hospital, Hohhot, Inner Mongolia 010017, China * | 010017 |
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| Abstract: |
| Objective The aim of this study was to enhance the treatment effect of tumor purified autogenous heat
shock protein 70-peptide complexes (HSP70-PCs) on HER-3-overexpressing breast cancer.
Methods In this study, we first studied the expression of HER-3 in breast cancer tissues and its
relationship with patient characteristics. We then purified HSP70-PCs from primary breast cancer cells with
different HER-2 and HER-3 expression profiles and determined the cytotoxicity of autogenous dendritic
cells (DCs) and CD8+ T cells induced by these complexes. Third, recombinant human HSP70-HER-3
protein complexes were used to inhibit the autogenous HSP70-PCs purified from HER-3–overexpressing
breast cancer cells, and the resulting immunological response was examined.
Results The results show that HSP70-PCs can be combined with recombinant HSP70-HER-3 protein
complexes to induce stronger immunological responses than autogenous HSP70-PCs alone and that these
treatments induce autogenous CD8+ T cell killing of HER-3-positive breast cancer cells.
Conclusion These findings provide a new direction for HSP70-DC-based immunotherapy for patients
with HER-3-overexpressing breast cancer. |
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