| Xiaojun Zhou,Baorui Lin,Yun Wang. Expression of mir-34c-5p and mir-150-5p in nasopharyngeal carcinoma and up-regulated expression after invasion and apoptosis of nasopharyngeal carcinoma cells. Oncol Transl Med, 2021, 7: 25-30. |
| miR-150-5p及miR-34c-5p在鼻咽癌的表达及其上调对鼻咽癌HNE1细胞凋亡及侵袭的影响 |
| Expression of mir-34c-5p and mir-150-5p in nasopharyngeal carcinoma and up-regulated expression after invasion and apoptosis of nasopharyngeal carcinoma cells |
| Received:June 18, 2020 Revised:February 22, 2021 |
| DOI：10.1007/s10330-020-0438-8 |
| 中文关键词: miR-150-5p；miR-34c-5p；鼻咽癌；细胞凋亡；侵袭 |
| 英文关键词: miR-150-5p; miR-34c-5p; nasopharyngeal carcinoma; apoptosis; invasion |
| 基金项目:国家自然科学基金81373698 |
| Author Name | Affiliation | E-mail | | Xiaojun Zhou* | Otolaryngology department,Zhongshan Hospital affiliated to Guangzhou University of Chinese Medicine | zd1232@126.com | | Baorui Lin | Otolaryngology department,Zhongshan Hospital affiliated to Guangzhou University of Chinese Medicine | | | Yun Wang | Pathology department,Zhongshan Hospital affiliated to Guangzhou University of Chinese Medicine | |
|
| Hits: 11400 |
| Download times: 14425 |
| 中文摘要: |
| 目的：研究miR-150-5p及miR-34c-5p在鼻咽癌的表达及其上调对HNE1细胞凋亡及侵袭能力的影响。方法：临床上采用基因芯片及RT-PCR技术检测30例鼻咽癌及鼻咽炎miR-150-5p及miR-34c-5p的表达。细胞实验通过脂质体转染方法将miR-150-5p及miR-34c-5p在鼻咽癌细胞HNE1进行转染，采用q R T - P C R 技术验证转染细胞中miR-150-5p及miR-34c-5p的表达水平；通过流式细胞术及Transwell实验观察miR-150-5p及miR-34c-5p过表达对细胞凋亡及侵袭能力的影响。结果：miR-150-5p在鼻咽癌及鼻咽炎表达分别为：0.165±0.092及1.062±0.280，miR-34c-5p在鼻咽癌及鼻咽炎表达量分别为0.417±0.220及1.385±0.739，鼻咽癌组织两个miR均呈现低表达。转染后has-miR-34c-5p及hsa-miR-150-5p的HNE1细胞凋亡率明显增加，分别为12.7%和7.6%，明显高于空白对照组（3.9%）；Transwell实验显示过表达 miR-150-5p与 miR-34c-5p后 HNE1细胞侵袭数分别为（32.00±2.00）及（28.33±2.08）个，细胞侵袭能力明显减弱，而空白对照组HNE1细胞侵袭能力维持在高水平（60.66±8.50）个，p＜0.01。结论：miR-150-5p及miR-34c-5p在鼻咽癌组织呈现低表达，其下调表达可能在鼻咽癌的发生和进展中发挥重要作用 |
| 英文摘要: |
| Objective MiRNAs are closely related to tumors, and we hypothesized there is specific miR expression
in nasopharyngeal carcinoma (NPC). We intended to investigate the expression of mir-34c-5p and mir-150-
5p in NPC and to investigate the effects of mir-34c-5p and mir-150-5p on apoptosis and invasion following
up-regulated expression in HNE1 NPC cells.
Methods MiR-34c-5p and miR-150-5p expression levels in 30 individual cases of NPC and nasopharyngitis
were detected with gene chip and qRT-PCR techniques. miR-34c-5p and miR-150-5p were transfected into
the NPC cell line HNE1 via liposomes. Their expression levels were detected with qRT-PCR, apoptosis was
evaluated by flow cytometry, and invasion ability was assessed via Transwell migration assay.
Results MiR-150-5p expression levels in NPC and nasopharyngitis were 0.165 ± 0.092 and 1.062 ±
0.280 respectively, and miR-34c-5p expression levels in NPC and nasopharyngitis were 0.417 ± 0.220 and
1.385 ± 0.739, respectively, which indicated miR-34c-5p and miR-150-5p were weakly expressed in NPC.
Apoptosis rates in HNE1 cells transfected by miR-34c-5p and miR-150-5p were increased, by 12.7% and
7.6%, respectively, which were significantly higher compared to blank control (3.9%). The Transwell assay
demonstrated that invasive HNE1 cell counts were 32.00 ± 2.00 and 28.33 ± 2.08, respectively, compared
to 60.66 ± 8.50 in the blank control (P < 0.001).
Conclusion MiR-34c-5p and miR-150-5p are lowly expressed in NPC, and their down-regulation may
be associated with NPC. |
|
View Full Text
Download reader HTML全文 |
| Close |