| Xiaojun Zhou,Baorui Lin,Yun Wang. Expression of mir-34c-5p and mir-150-5p in nasopharyngeal carcinoma and up-regulated expression after invasion and apoptosis of nasopharyngeal carcinoma cells. Oncol Transl Med, 2021, 7: 25-30. |
| Expression of mir-34c-5p and mir-150-5p in nasopharyngeal carcinoma and up-regulated expression after invasion and apoptosis of nasopharyngeal carcinoma cells |
| Received:June 18, 2020 Revised:February 22, 2021 |
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| KeyWord:miR-150-5p; miR-34c-5p; nasopharyngeal carcinoma; apoptosis; invasion |
| Author Name | Affiliation | E-mail | | Xiaojun Zhou | Otolaryngology department,Zhongshan Hospital affiliated to Guangzhou University of Chinese Medicine | zd1232@126.com | | Baorui Lin | Otolaryngology department,Zhongshan Hospital affiliated to Guangzhou University of Chinese Medicine | | | Yun Wang | Pathology department,Zhongshan Hospital affiliated to Guangzhou University of Chinese Medicine | |
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| Abstract: |
| Objective MiRNAs are closely related to tumors, and we hypothesized there is specific miR expression
in nasopharyngeal carcinoma (NPC). We intended to investigate the expression of mir-34c-5p and mir-150-
5p in NPC and to investigate the effects of mir-34c-5p and mir-150-5p on apoptosis and invasion following
up-regulated expression in HNE1 NPC cells.
Methods MiR-34c-5p and miR-150-5p expression levels in 30 individual cases of NPC and nasopharyngitis
were detected with gene chip and qRT-PCR techniques. miR-34c-5p and miR-150-5p were transfected into
the NPC cell line HNE1 via liposomes. Their expression levels were detected with qRT-PCR, apoptosis was
evaluated by flow cytometry, and invasion ability was assessed via Transwell migration assay.
Results MiR-150-5p expression levels in NPC and nasopharyngitis were 0.165 ± 0.092 and 1.062 ±
0.280 respectively, and miR-34c-5p expression levels in NPC and nasopharyngitis were 0.417 ± 0.220 and
1.385 ± 0.739, respectively, which indicated miR-34c-5p and miR-150-5p were weakly expressed in NPC.
Apoptosis rates in HNE1 cells transfected by miR-34c-5p and miR-150-5p were increased, by 12.7% and
7.6%, respectively, which were significantly higher compared to blank control (3.9%). The Transwell assay
demonstrated that invasive HNE1 cell counts were 32.00 ± 2.00 and 28.33 ± 2.08, respectively, compared
to 60.66 ± 8.50 in the blank control (P < 0.001).
Conclusion MiR-34c-5p and miR-150-5p are lowly expressed in NPC, and their down-regulation may
be associated with NPC. |
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