Yue Li,Yuling Bai,Yan Qi,Chang Cai,Ying Liao,Xiuzhu Liu,Pengcheng He. Expression and role of PTV1 lncRNA in glioma cells progression. Oncol Transl Med, 2021, 7: 51-58.
Expression and role of PTV1 lncRNA in glioma cells progression
Received:June 07, 2020  Revised:April 23, 2021
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KeyWord:glioma; miRNA-203a; long-chain noncoding RNA; transcription factor PTV1 lncRNA
Author NameAffiliationE-mail
Yue Li Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China dengdeng9988@163.com 
Yuling Bai Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China  
Yan Qi Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China  
Chang Cai Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China  
Ying Liao Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China  
Xiuzhu Liu Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China  
Pengcheng He Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China yueyue52887@sina.com 
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Abstract:
      Objective The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and the mechanism of its interaction with miR-203a. Methods U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively. The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude mice were divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, then miR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumorrelated proteins. Results The relative expression of PTV1 in gliomas was significantly higher than that in normal brain tissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1 significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones; overexpression of rPTV1 significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual Luciferase Reporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with a miR-203a mimic, the expression of SP2 decreased; and when U251 cells were treated with a miR-203a inhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and the proliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showed the opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice was significantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administration of the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increased significantly, while u251-ov mice showed the opposite trend. Conclusion lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order to downregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be a new biomarker and therapeutic target for glioma.
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