| Yue Li,Yuling Bai,Yan Qi,Chang Cai,Ying Liao,Xiuzhu Liu,Pengcheng He. Expression and role of PTV1 lncRNA in glioma cells progression. Oncol Transl Med, 2021, 7: 51-58. |
| Expression and role of PTV1 lncRNA in glioma cells progression |
| Received:June 07, 2020 Revised:April 23, 2021 |
| View Full Text View/Add Comment Download reader |
| KeyWord:glioma; miRNA-203a; long-chain noncoding RNA; transcription factor PTV1 lncRNA |
| Author Name | Affiliation | E-mail | | Yue Li | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | dengdeng9988@163.com | | Yuling Bai | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | | | Yan Qi | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | | | Chang Cai | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | | | Ying Liao | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | | | Xiuzhu Liu | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | | | Pengcheng He | Department of Neurosurgery, Mianyang Central Hospital, Mianyang 621000, China | yueyue52887@sina.com |
|
| Hits: 3465 |
| Download times: 3777 |
| Abstract: |
| Objective The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and the
mechanism of its interaction with miR-203a.
Methods U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively.
The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude mice
were divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, then
miR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumorrelated
proteins.
Results The relative expression of PTV1 in gliomas was significantly higher than that in normal brain
tissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1
significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones; overexpression of rPTV1
significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual Luciferase
Reporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with a
miR-203a mimic, the expression of SP2 decreased; and when U251 cells were treated with a miR-203a
inhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and the
proliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showed
the opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice was
significantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administration
of the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increased
significantly, while u251-ov mice showed the opposite trend.
Conclusion lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order to
downregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be a
new biomarker and therapeutic target for glioma. |
| Close |
|
|
|