| Wei-Guo Xu,Xiang-mei Liu. Silencing Neuropilin 1 gene reverses TGF-β1-induced epithelial mesenchymal transition in HGC-27 gastric cancer cell line. Oncol Transl Med, 2020, 6: 258-265. |
| 基因沉默Neuropilin1逆转TGF-β1诱导的胃癌HGC-27细胞上皮间质转化 |
| Silencing Neuropilin 1 gene reverses TGF-β1-induced epithelial mesenchymal transition in HGC-27 gastric cancer cell line |
| Received:March 26, 2020 Revised:October 29, 2020 |
| DOI：10.1007/s10330-020-0412-2 |
| 中文关键词: Neuropilin1；上皮间质转化；胃癌；转化生长因子-β1 |
| 英文关键词: Neuropilin1 (NRP1); epithelial-mesenchymal transition (EMT); gastric cancer; transforming ?growth fqactor-β1 |
| 基金项目:引进留学人员资助项目 (CY201721). |
| Author Name | Affiliation | E-mail | | Wei-Guo Xu* | the Affiliated Hospital of North China University of Science and Technology | mimimay860@gmail.com | | Xiang-mei Liu | the Affiliated Hospital of North China University of Science and Technology | |
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| 中文摘要: |
| 目的: 研究NRP1有助于转化生长因子β1（TGF-β1）诱导的胃癌HGC-27细胞上皮间质转化（EMT）及其作用机制。
方法: 用TGF-β1刺激胃癌HGC-27细胞诱导EMT模型。NRP1-siRNA稳定转染胃癌HGC-27细胞沉默NRP1的表达。划痕及Transwell实验分析细胞的迁移与侵袭转移。qRT-PCR和Western blot检测NRP1和EMT相关蛋白(E cadherin, vimentin, snail)的表达水平。使用方差分析进行多组之间的比较，Student t检验进行两组间的比较。*P <0.05，差异具有统计学意义。
结果: TGF-β1刺激诱导胃癌HGC-27细胞呈EMT改变，促进细胞的迁移和侵袭转移。基因沉默NRP1将抑制胃癌HGC-27细胞的迁移和侵袭转移。TGF-β1刺激诱导胃癌HGC-27细胞后基因沉默NRP1，细胞的迁移和侵袭转移能力下调并部分逆转EMT过程。
结论: NRP-1可能是胃癌的潜在有价值的治疗靶点。 |
| 英文摘要: |
| Objective?The aim of this study was to determine Neuropilin 1 (NRP1) contribution to transforming growth factor β1 (TGF-β1)-induced epithelial mesenchymal transition (EMT) of HGC-27 gastric cancer cells and study its mechanism.
Methods?In this study, TGF-β1 was used to induce EMT in HGC-27 cells. Further, these cells were stably transfected with siRNA targeting NRP1. Wound healing and transwell assays were used to measure cell migration and invasion, respectively. NRP1 and EMT markers were measured using quantitative real time reverse transcription polymerase chain reaction and western blotting.
Results?Exposure of TGF-β1 conferred a fibroblastic-like shape to cancer cells and significantly increased the expression of NRP1 in HGC-27 cells. TGF-β1 subsequently promoted migration and invasion of HGC-27 cells. Furthermore, silencing NRP1 inhibited the invasion and migration of TGF-β1-induced cells undergoing EMT.
Conclusion?Silencing NRP1 can inhibit cell migration, invasion, and metastasis and reverse the TGF-β1-induced EMT process of gastric cancer. |
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