| Yanwei Gao,Xia Chen,Weishi Gao,Xiangji Lu,Lin Peng. A study on melanoma treatment using dendritic cells loaded with antigens purified from melanoma cell lines. Oncol Transl Med, 2020, 6: 21-25. |
| A study on melanoma treatment using dendritic cells loaded with antigens purified from melanoma cell lines |
| Received:December 08, 2019 Revised:December 08, 2019 |
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| KeyWord:heat shock protein 70 peptide complexes; dendritic cells; CIK cells; melanoma; cellular immunotherapy |
| Author Name | Affiliation | E-mail | | Yanwei Gao | Department of surgical oncology,Inner Mongolia People’s Hospital,Hohhot,Inner Mongolia | gaoyw0518@163.com | | Xia Chen | Department of blood components preparation,Inner Mongolia Red Cross Blood Center,Hohhot,Inner Mongolia | | | Weishi Gao | Department of surgical oncology,Inner Mongolia People’s Hospital,Hohhot,Inner Mongolia | | | Xiangji Lu | Department of emergency,Inner Mongolia Armed Police Hospital,Hohhot,Inner Mongolia | | | Lin Peng | Department of gastroenterology, Inner Mongolia People’s Hospital | penglinlaoshi@126.com |
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| Abstract: |
| Objective The aim of this study was to purify effective tumor peptide complexes from human melanoma
cell lines to enhance the treatment effects on melanoma.
Methods We purified heat shock protein 70 (HSP70)-peptide complexes (PCs) from human melanoma
cell lines A375, A875, M21, M14, WM-35, and SK-HEL-1. We named the purified product as M-HSP70-
PCs and determined its immunological activities. Autologous HSP70-PCs purified from primary tumor
cells of melanoma patients (9 cases) were used as controls. These two tumor antigenic complexes were
loaded into dendritic cells (DCs) and used to stimulate an antitumor response against tumor cells in the
corresponding patients.
Results Mature DCs pulsed with M-HSP70-PCs stimulated autologous T cells to secrete the same levels
of type I cytokines as the autologous HSP70-PCs. Moreover, DCs pulsed with M-HSP70-PCs endued CIK
cells with an equal ability as autologous HSP70-PCs to kill melanoma cells in the patients.
Conclusion M-HSP70-PCs may be used as an efficient and generalized tumor antigen in the treatment
of DC-based malignant melanoma. |
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