| Weigang cao,Baoan Qiu. Experimental study on the relationship between traumatic stress and tumor growth, proliferation, and metastasis*. Oncol Transl Med, 2020, 6: 52-56. |
| 创伤应激与肿瘤生长、增殖和转移的实验研究 |
| Experimental study on the relationship between traumatic stress and tumor growth, proliferation, and metastasis* |
| Received:October 16, 2019 Revised:April 13, 2020 |
| DOI：10.1007/s10330-019-0385-5 |
| 中文关键词: 应激反应；大鼠；肿瘤；烧伤 |
| 英文关键词: stress response; Wistar rats; tumor; scalding |
| 基金项目: |
| Author Name | Affiliation | E-mail | | Weigang cao | Yantai Affiliated Hospital of Binzhou Medical University | cwg929@sina.com | | Baoan Qiu* | The Sixth Medical Center of PLA General Hospital | luckqiu@medmail.com |
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| 中文摘要: |
| 目的探讨创伤性应激与肿瘤生长、增殖、转移的关系。
使用Wistar大鼠的方法。采用滚烫法作为诱发创伤性应激的有害因素。大鼠随机分为对照组、轻度烫伤组和重度烫伤组，每组14只。用wistar大鼠对walker-256细胞线进行培养，生成肿瘤腹水。对腹水组织中的肿瘤细胞进行培养，建立大鼠皮下异种移植模型。7天后，他们的背部分别受到10%和15%的烧伤。在不同时间从大鼠的尾静脉抽血，以检测血液皮质醇、IL-1β和TNF-α水平的变化。14天后采集病理标本，进行免疫组织化学检查血管内皮生长因子、增殖细胞核抗原、e-cadherine和Vimentin。
结果大鼠经烫伤后，皮质醇、IL-1β和TNF-α水平均明显高于对照组，差异有统计学意义。14天后进行肿瘤检查。肿瘤大小变化显示对照组（0.593±0.195 CM3）和轻度烫伤组（0.782±0.344 CM3）肿瘤体积无明显差异。但是，在七度烧伤组(1.806±0.838厘米)3,肿瘤体积明显大于对照组和多发性烧伤组(p<0.05)。肿瘤组织免疫组化结果表明，对照组、轻度烫伤组和重度烫伤组表达pcna的细胞比例分别为57.1%、71.4%和85.7%，两组间差异具有统计学意义。同时，轻度和重度烫伤组的蔬菜阳性细胞数量明显高于对照组，且差异有统计学意义（p<0.05）。重烫伤组肿瘤组织中e-钙粘蛋白阳性细胞的比例明显低于对照组和轻度烫伤组。Vimentin在肿瘤组织中表现出相反的趋势，差异有统计学意义（p<0.05）。
结论烫伤引起的不同程度的创伤性反应可引起相应的身体应激反应。炎症介质的释放，肿瘤组织中的vegf，pcna和Vimentin的增加，e-钙粘蛋白的减少导致了肿瘤组织的生长和转移的改变。创伤性应激与肿瘤生长、增殖和转移有关。 |
| 英文摘要: |
| Objective This study aimed to investigate the relationship between traumatic stress and tumor growth,
proliferation, and metastasis.
Methods A scalding method was used as an injurious factor to induce traumatic stress in Wistar rats. The
rats were randomly divided into three groups—the control group, mild-scald group, and severe-scald group,
with 14 rats in each group. Wistar rats were used to subculture the Walker-256 cell line for the generation
of tumor ascites. Tumor cells from the ascites were cultured and used to establish a rat subcutaneous
xenograft model. After 7 days, the mild-burn group and the severe-burn group were subjected burns to
10% and 15% of their backs, respectively. Blood was taken from the tail vein of rats at different times to
detect changes in blood cortisol, IL-1β, and TNF-α levels. Pathological specimens were collected 14 days
later, and immunohistochemistry was performed to examine vascular endothelial growth factor (VEGF),
proliferating cell nuclear antigen (PCNA), E-cadherin, and vimentin.
Results Cortisol, IL-1β and TNF-α levels were significantly higher in the scalding groups than in the
control group. Tumor examination was performed after 14 days. The changes in tumor size showed
that the tumor volume in the control group (0.593 ± 0.195 cm3) and the mild-scald group (0.782 ± 0.344
cm3) were not significantly different. However, the tumor volume was significantly larger in the severeburn
group (1.806 ± 0.838 cm3) than in the control and the mild-burn groups(P < 0.05). Tumor tissue
immunohistochemistry showed that the percentage of cells expressing PCNA in the control group, mildscald
group, and severe-scald group was 57.1%, 71.4% and 85.7%, respectively, and the differences
among the groups were statistically significant. The number of VEGF-positive cells in the mild- and severescald
groups was significantly higher than those of the control group (P < 0.05). The number of E-cadherinpositive
cells in the tumor tissues was significantly lower in the severe-scald group than that in the control
and mild-scald groups. Vimentin showed the opposite trend in the tumor tissue, and the differences were
statistically significant (P < 0.05).
Conclusion Different degrees of a traumatic response in tissues caused by scalding can cause a
corresponding stress response in the body. The release of inflammatory mediators; increase in VEGF,
PCNA and vimentin in the tumor tissue; and decrease in E-cadherin lead to a change in tumor tissue growth
and metastasis. Traumatic stress is associated with tumor growth, proliferation, and metastasis. |
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