| Lu Han,Yang-Yang Shen,Wen-Hua Zhao,Bao-Yong Sun,Xin Zhang,Kai Cui,Lei Zhou,Sheng Li. Expression of CXCL12-CXCR4 in osteosarcoma and its correlation with angiogenesis. Oncol Transl Med, 2017, 3: 254-259. |
| Expression of CXCL12-CXCR4 in osteosarcoma and its correlation with angiogenesis |
| Received:December 05, 2016 Revised:December 13, 2017 |
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| KeyWord:osteosarcoma; stromal cell-derived factor-1 (CXCL12); chemokine receptors-4 (CXCR4) angiogenesis; pulmonary metastatics |
| Author Name | Affiliation | Postcode | | Lu Han | School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250117, China | 250117 | | Yang-Yang Shen | Department of Anesthesiology, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | | | Wen-Hua Zhao | Department of Tumor Minimally Invasive Surgery, Qianfo Hill Hospital of Shandong Province, Jinan 250014, China | | | Bao-Yong Sun | Department of Bone & Soft Tissue Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | | | Xin Zhang | Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University | | | Kai Cui | Department of Hepatobiliary Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | | | Lei Zhou | Department of Bone & Soft Tissue Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China | cc | | Sheng Li | School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250117, China | |
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| Abstract: |
| Objective: The expression of CXCL12 (stromal cell-derived factor-1)-CXCR4 (chemokine receptors-4) in
osteosarcoma and its role in angiogenesis were examined.
Methods: The expression of CXCR4 and CXCL12 in 40 cases of osteosarcoma was detected by
immunohistochemistry and real-time fluorescence quantitative PCR. The expression of CD34 in
osteosarcoma was detected by immunohistochemistry. Morphometric image analysis was performed
to measure microvessel density (MVD). Additionally, the relationship between CXCL12 and CXCR4
expression and MVD of osteosarcoma and pulmonary metastasis were analyzed.
Results: The positive rates of CXCL12 and CXCR4 protein expression in osteosarcoma were 40.0%
(16/40) and 60.0% (24/40), respectively. Fluorescence quantitative real-time PCR indicated that the
expression level of CXCR4 mRNA in pulmonary metastatic osteosarcoma was higher than that in nonpulmonary
metastatic osteosarcoma (P < 0.01). The level of MVD in pulmonary metastatic osteosarcoma
was higher than that in non-pulmonary metastatic osteosarcoma (P < 0.01).
Conclusion: The expression level of CXCR4 was significantly associated with pulmonary metastasis and
angiogenesis of osteosarcoma. |
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