| Xuli Yang,Li Wang,Wenli Sai,Yin Cai,Juanjuan Gu,Xin Chen,Dengfu Yao. Clinicopathological features of hypoxia-inducible factor-1α and vascular endothelial growth factor expression in patients with lung cancer. Oncol Transl Med, 2016, 2: 261-267. |
| Clinicopathological features of hypoxia-inducible factor-1α and vascular endothelial growth factor expression in patients with lung cancer |
| Received:January 16, 2016 Revised:December 02, 2016 |
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| KeyWord:lung cancer; hypoxia-inducible factor-1α (HIF-1α); nucleic acid metabolism; enzyme-linked immunosorbent assay (ELISA); diagnosis |
| Author Name | Affiliation | Department | | Xuli Yang | The Affiliated Hospital of Nantong University, Nantong 226001, China | Research Center of Clinical Medicine | | Li Wang | Medical School of Nantong University, Nantong 226001, China | Center of Medical Informatics | | Wenli Sai | The Affiliated Hospital of Nantong University, Nantong 226001, China | Research Center of Clinical Medicine | | Yin Cai | The Affiliated Hospital of Nantong University, Nantong 226001, China | Department of Oncology | | Juanjuan Gu | The Affiliated Hospital of Nantong University, Nantong 226001, China | Research Center of Clinical Medicine | | Xin Chen | Nantong University, Nantong 226001, China | School of Life Science | | Dengfu Yao | Research Center of Clinical Medicine, Affiliated Hospital of Nantong University
| Research Center of Clinical Medicine |
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| Abstract: |
| Objective The aim of the study was to investigate the clinicopathological characteristics of hypoxiainducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression in patients with lung
cancer.
Methods Cancerous and noncancerous tissues were collected post-operation from 115 patients with lung
cancers by the self-control method. Total RNA was extracted from the lung tissues. The status of tissue HIF-
1α expression and intercellular distribution was observed by immunochemistry using a tissue microarray.
The expression levels of circulating HIF-1α and VEGF were detected by enzyme-linked immunosorbent
assay (ELISA).
Results The expression of serum HIF-1α [(138.3 ± 28.8) μg/L] in the group of patients with lung cancer
was significantly higher (P < 0.01) than that in the group of patients with pneumonia [(58.8 ± 14.5) μg/L] and
the control group of patients [(24.1 ± 3.3) μg/L]. There was a strong positive correlation of serum HIF-1α
levels (r = 0.937, P < 0.01) with serum VEGF levels. The specific concentration of total RNA [(1.52 ± 1.14)
μg/mg wet lung tissues] in the cancerous tissues was significantly higher (t = 8.494, P < 0.001) than that in
the noncancerous tissues [(0.58 ± 0.33) μg/mg]. The clinicopathological features of HIF-1α expression in
lung cancer tissues revealed a significant relationship between positive HIF-1α expression and patient sex
(χ2 = 4.494, P = 0.034), tumor size (χ2 = 4.679, P = 0.031), differentiation degree (χ2= 8.846, P = 0.012),
and presence of lymphatic node metastasis (χ2= 6.604, P = 0.037).
Conclusion Abnormal HIF-1α expression in lung cancer is closely related with nucleic acid metabolism
and angiogenesis, and it may be helpful in the diagnosis and identification of lung cancer. |
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