| Wei Zhuang,Limin Lun. Preliminary research on dendritic cells loaded with resistant breast cancer antigens in breast cancer-bearing nude mice. Oncol Transl Med, 2015, 1: 120-124. |
| Preliminary research on dendritic cells loaded with resistant breast cancer antigens in breast cancer-bearing nude mice |
| Received:January 16, 2015 Revised:February 10, 2015 |
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| KeyWord:ATP-TCA; breast cancer; dendritic cell; nude mice; resistance |
| Author Name | Affiliation | E-mail | | Wei Zhuang | Medical College of Qingdao University | zw2008006@163.com | | Limin Lun | The Affiliated Hospital of Qingdao University | lunlmqd@163.com |
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| Abstract: |
| Objective The aim of the study was to investigate the inhibitory effects of dendritic cells (DCs) loaded
with resistant breast cancer antigens on breast cancer in nude mice.
Methods A single-cell suspension was prepared from a primary breast cancer and chemotherapeutic
drugs were screened using the ATP-PCA susceptibility testing system. Cancer cells were treated with 1/10
× IC50, 1/5 × IC50, 1/2 × IC50, 1 × IC50, and 2 × IC50 medium until their growth became steady in the 2 × IC50
medium. Peripheral blood mononuclear cells (PBMCs) were obtained from the peripheral blood of patients
with leukapheresis. The obtained adherent cells were induced by granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) to generate DCs, which carried resistant strain cell lysis
compounds or non-treated cancer cell lysis compounds. The former mature DCs carried resistant breast
tumor antigens. A breast tumor-bearing nude mouse model was established with these resistant strains and
the mice were randomly divided in three groups. The mice in the treatment group were injected with DCs
loaded with resistant breast cancer antigens. The control group consisted of mice injected with DCs loaded
with primary tumor cell antigens and the blank group consisted of mice injected with the same volume of
normal saline. Changes in the cancers were observed.
Results After treatment with the effector cells, the cancer volume and weight were significantly different
to those before treatment in every group of mice (P < 0.05). The tumor volume in the blank group was the
largest (3.362 ± 0.068 cm3) and the tumor weight was 637.50 ± 59.398 mg. Compared to the blank group,
the tumor volume in the experimental group was the smallest (1.273 ± 0.071 cm3) and the tumor weight
was 206.81 ± 32.711 mg.
Conclusion DCs loaded with resistant breast cancer antigens demonstrated a significant inhibition effect
on the cancers of breast tumor-bearing nude mice. |
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