| Yan Shi,Yana Li,Naie Li,Min Yu,Dong Wang,Lijun Kong. Ethanolic extract of propolis induces apoptosis of HL-60 cells in vitro. Oncol Transl Med, 2016, 2: 34-38. |
| Ethanolic extract of propolis induces apoptosis of HL-60 cells in vitro |
| Received:December 29, 2014 Revised:October 22, 2015 |
| View Full Text View/Add Comment Download reader |
| KeyWord:propolis; leukemia; apoptosis; Bcl-2/Bax |
| Author Name | Affiliation | E-mail | | Yan Shi | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Yana Li | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Naie Li | Department of Diagnostics, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Min Yu | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Dong Wang | Department of Histology and Embryology, Binzhou Medical College, Yantai 264003, China | sheeryarn@126.com | | Lijun Kong | Department of Biochemistry, Binzhou Medical College, Yantai 264003, China | kong_lijun@163.com |
|
| Hits: 7272 |
| Download times: 8685 |
| Abstract: |
| Objective: The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells.
Methods: HL-60 cells were treated for 24, 48, 72 h with various concentrations ethanolic extracts of propolis (0, 50, 100, and 200 μg/mL). The proliferation of HL-60 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-iphenyltetrazolium bromide (MTT) assay. Subsequently, Hochest 33258 staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were used to test the apoptosis of HL-60 cells. We observed the expression levels of Bax and Bcl-2 in HL-60 cells by immunohistochemistry.
Results: MTT assay showed that various concentrations of ethanolic extract of propolis had significant inhibitory effect on HL-60 cell proliferation (P < 0.05). Typical morphologic changes could be observed by fluorescence microscope and TUNEL. By immunohistochemistry, we found the expression level of Bax was up-regulated, whereas that of Bc1-2 was down-regulated (P < 0.05).
Conclusion: Ethanolic extract of propolis inhibits leukemia cell proliferation and induces apoptosis in vitro. Its mechanism may be related to the regulation of Bax and Bcl-2 expression and up-regulation of Bcl-2/Bax ratio. |
| Close |
|
|
|