| Yonghong Zhang,Yongxiang Li. A traditional Chinese medicine Huaier triggers G1 cell cycle arrest and apoptosis through cyclins-CDKs-CKIs machinery in MOLT4 cells. Oncol Transl Med, 2014, 13: 603-608. |
| A traditional Chinese medicine Huaier triggers G1 cell cycle arrest and apoptosis through cyclins-CDKs-CKIs machinery in MOLT4 cells |
| Received:September 26, 2014 Revised:December 13, 2014 |
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| KeyWord:Chinese medicine; apoptosis; cell cycle arrest; MOLT4 |
| Author Name | Affiliation | E-mail | | Yonghong Zhang | yong-hong zhang: first author,medical doctor,chief physician,E-mail:@qqcom Yong-xiang Li: corresponding author | 379847410@qq.com | | Yongxiang Li | Anhui Medical University affiliated First Hospital | 379847410@qq.com |
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| Abstract: |
| Objective: The purpose of the study was to study the effect of Huaier, a traditional Chinese medicine, on the
cell cycle adjustment in MOLT4 cells in vitro. Methods: We used MTT assay to test cell viability, flow cytometry to detect cell
cycle and apoptosis and western blot to examine the expression of cell-cycle and apoptotic proteins in MOLT4 cells induced by Huaier. Results: Huaier could reduce the viability of MOLT4 cell by inducing G1 arrest and apoptosis. The induction of apoptosis after treatment with Huaier for 24 h was demonstrated in a dose- and time-dependent manner by flow cytometry analysis. G1 arrest induced by Huaier was modulated through the increased expression of Cdki proteins (p21cip/waf1 and p27kip1) with a simultaneous decrease in Cdk2, Cdk4, Cdk6, cyclin D1 and cyclin E expression. Huaier also induced Bax and Bcl-2 expression and activation of Caspase-3. Conclusion: It is firstly demonstrated that Huaier can inhibit proliferation of MOLT4 cells via G1 arrest and apoptosis. These results suggest that Huaier is a cell-cycle anti-cancer drug. |
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