| Yuezhi Zhang,Hanchen Liu,Mengwen Li,Lijun Kong. The Effect and significance on Akt and PTEN Expression in melanoma B16 Cells with chamaejasme extract. Oncol Transl Med, 2014, 13: 600-602. |
| The Effect and significance on Akt and PTEN Expression in melanoma B16 Cells with chamaejasme extract |
| Received:September 22, 2014 Revised:October 17, 2014 |
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| KeyWord:malignant melanoma; chamaejasme extract; Akt chamaejasme; PTEN; immunocytochemistry |
| Author Name | Affiliation | E-mail | | Yuezhi Zhang | Biochemistry and molecular biology laboratory, Binzhou Medical College, Yantai, Shandong 264003, China | jingxuanlemontre@tom.com | | Hanchen Liu | Department of radiotherapy , The PLA 107 hospital, Yantai, Shandong 264002, China | 49463515@qq.com | | Mengwen Li | Department of radiotherapy , The PLA 107 hospital, Yantai, Shandong 264002, China | yousun_mo@163.com | | Lijun Kong | Biochemistry and molecular biology laboratory, Binzhou Medical College, Yantai, Shandong 264003, China | kong_lijun@163.com |
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| Abstract: |
| Objective: The aim of this study is to investigate the effect on Akt and PTEN expression and the mechanism of proliferation and apoptosis of melanoma B16 cells treated with chamaejasme extract. Methods: The expressions of Akt and PTEN of B16 cells treated with different concentrations of chamaejasme extract were detected with immunohistochemical method, cell apoptosis index (AI) was calculated with in situ labeling method. Results: Akt expressions of B16 cells treated with different concentrations of chamaejasme extract were significantly lower than the control group, while the PTEN expressions significantly upregulated, and the effects appeared to be dose-related(P<0.05). The Akt/AI of B16 cells treated with different concentrations of chamaejasme extract was significantly lower than the control group, all the parameters had extremely difference(F=24.58,P<0.05. Conclusion: Chamaejasme extract could inhibit proliferation and induce apoptosis of malignant melanoma B16 cells by down-regulating the expression of Akt and up-regulating the expression of PTEN. |
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