| Dengfu Yao. Diagnostic and monitoring Values of Circulating AFP mRNA and HS-GGT for Hepatocellular Carcinoma. Oncol Transl Med, 2014, 13: 457-463. |
| Diagnostic and monitoring Values of Circulating AFP mRNA and HS-GGT for Hepatocellular Carcinoma |
| Received:August 21, 2014 Revised:October 06, 2014 |
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| KeyWord:Hepatocellular carcinoma; AFP mRNA; Hepatoma-specific gamma- glutamyl transferase; Diagnosis; Metastasis |
| Author Name | Affiliation | E-mail | | Dengfu Yao | Research Center of Clinical Medicine, Affiliated Hospital of Nantong University
| yaodf@ahnmc.com |
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| Abstract: |
| BACKGROUD The early finding of hepatocellular carcinoma (HCC) and monitoring of its metastasis are of the utmost importance. The objectives of the study were to investigate the values of circulating hepatoma-specific γ-glutamyl transferase (HS-GGT) fraction and α-fetoprotein mRNA (AFP-mRNA) from peripheral blood mononuclear cells (PBMCs) in diagnosis or monitoring metastasis of HCC.
METHODS Total RNA was extracted from hepatomas or PBMCs of patients, synthesized to AFP-cDNA through random primers and reverse transcriptase, amplified by using a nested PCR assay, and confirmed by sequencing. Simultaneity, the HS-GGT activities were quantitatively determined in sera of patients. The comprehensive assessments of two markers were investigated in HCC patients.
RESULTS The amplified fragments of AFP-mRNA were identical to original designed ones (159 bp) and confirmed by sequencing. The lowest sensitivity was 2 ng/L of total RNA. The incidences of AFP- mRNA and HS-GGT were 60.4 % (113 of 187) and 84.5 % (158 of 187) in the HCC group, and their significantly higher (P < 0.01) than that in any of other groups. The high frequencies of HS-GGT and AFP-mRNA were 78.2 % and 56.4 % in the HCC patients with AFP less than 50 ng/mL. All positive of AFP-mRNA was found in HCC patients with extrahepatic metastasis. No significant correlation was found between tumor size and AFP-mRNA or HS-GGT. The total incidence of combined AFP-mRNA and HS-GGT detection was 93.6 % for HCC diagnosis.
CONCLUSION The abnormal HS-GGT and presence of AFP-mRNA are specific markers for HCC diagnosis or differentiation, especially the former for early finding and the later for monitoring metastasis of HCC. |
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