| Lilin Hang,Min Zhang,Fanliang Meng,Mei Zhong,Jing Li. ZNF217 expression correlates with the biological behavior of human ovarian cancer cells. Oncol Transl Med, 2014, 13: 539-544. |
| ZNF217 expression correlates with the biological behavior of human ovarian cancer cells |
| Received:August 05, 2014 Revised:October 23, 2014 |
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| KeyWord:ovarian cancer; zinc-finger protein 217 (ZNF217) gene; gene expression; proliferation; invasion; tumor metastasis |
| Author Name | Affiliation | E-mail | | Lilin Hang | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515 | 352795915@qq.com | | Min Zhang | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Fanliang Meng | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Mei Zhong | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | | | Jing Li | Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China | lijing7405@126.com |
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| Abstract: |
| Objective: The aim of the study was to investigate the correlation of zinc-finger protein 217 (ZNF217) gene expression with the biological behavior of human ovarian cancer HO-8910 cells. Methods: The expression of ZNF217 in ovarian carcinoma cell lines was detected by RT-PCR and Western blot, respectively. The biological behaviors of the transfectants were investigated by MTT, in vitro Boyden chamber and in vivo invasion assay, respectively. Results: RT-PCR and Western blotting revealed that transfection of ZNF217 into the HO-8910 cells significantly increased their proliferation along with markedly enhanced in vitro and in vivo invasion and metastatic abilities. MTT assay showed that the proliferation ability of pEGFPN1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells and HO-8910 cells (P < 0.001). The Boyden chamber assay showed that the numbers of migrating pEGFP-N1-ZNF217/HO-8910, pEGFP-N1/ HO-8910 and HO-8910 cells were (141.25 ± 13.91) cells /200 × field, (82.50 ± 11.73) cells /200 × field and (81.75 ± 12.12) cells /200 × field, respectively, with a significant difference between them (F = 29.274, P < 0.001). The nude mouse experiment showed that the in vivo tumor formation ability of pEGFP-N1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells (P < 0.001). Conclusion: Based on these clinical and laboratory observations, we conclude that ZNF217 may contribute to ovarian cancer invasion and metastasis, and associated with worse clinical outcomes. We evaluated ZNF217’s role as a biomarker of ovarian carcinogenesis and tumor progression in patient samples and explored possible molecular mechanisms in promoting tumor growth and invasion. |
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