| Yuxuan Che,Xiuhua Sun,Chaomei Huang,Jinbo Zhao. Influence of Tamoxifen or the combination of Tamoxifen and Cisplatin on the growth of human lung adenocarcinoma A549 cells. Oncol Transl Med, 2014, 13: 523-527. |
| Influence of Tamoxifen or the combination of Tamoxifen and Cisplatin on the growth of human lung adenocarcinoma A549 cells |
| Received:July 15, 2014 Revised:October 21, 2014 |
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| KeyWord:Tamoxifen; Cisplatin (DDP); chemosensitization; A549 cells |
| Author Name | Affiliation | E-mail | | Yuxuan Che | The Second Affiliated Hospital of Dalian Medical University | cheyuxu_an_ok@126.com | | Xiuhua Sun | The Second Affiliated Hospital of Dalian Medical University | | | Chaomei Huang | The Second Affiliated Hospital of Dalian Medical University | | | Jinbo Zhao | The Second Affiliated Hospital of Dalian Medical University | zhaojinbo66@sina.com |
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| Abstract: |
| Objective: The experiment aims to investigate the influence of Tamoxifen and the combination of Tamoxifen and Cisplatin (DDP) on the growth of human lung adenocarcinoma A549 cells. Methods: We treated human lung adenocarcinoma A549 cells with different concentrations of Tamoxifen, DDP and combination of DDP and Tamoxifen with non-toxicity for 72 h. Then we calculated the inhibition rate through MTT approach and detected the apoptosis rate by flow cytometry. The statistical analysis was performed with SPSS 13.0 software and statistical differences were determined by one-way ANOVA. The data were expressed as the mean ± standard deviation and all experiments were performed in three times. The value of P < 0.05 was considered to indicate a statistically significant difference. Results: 1. The inhibition rates of Tamoxifen with 2.5 μmol/L, 5 μmol/L, 10 μmol/L and 20 μmol/L on the growth of the A549 cells were 18.7%, 25.8%, 54% and 98.8%, respectively (P = 0.000). Tamoxifen with concentration of 1 μmol/L has no obvious cytoxicity on the A549 cells (P > 0.05). 2. As the increase concentration of Tamoxifen, the S stage and G2/M of the A549 cells decreased while the G0/G1 increased. The apoptosis rate of Tamoxifen with 0 μmol/L, 0.1 μmol/L, 1 μmol/L and 10 μmol/L on the A549 cells were 6.51%, 8.91%, 17.97% and 42.7%, respectively. 3. The inhibition rates of combination of Tamoxifen with 1 μmol/L and DDP with 1.25 μg/mL, 2.5 μg/mL, 5 μg/mL, 10 μg/mL and 20 μg/mL on the A549 cells were 40.4%, 54.4%, 72.9%, 86.1% and 92.4%, respectively (P < 0.05). Conclusion: Tamoxifen can inhibit the proliferation of human lung adenocarcinoma A549 cells and induce the apoptosis of the A549 cells. The combination of Tamoxifen with non-toxicity and DDP can improve the sensitivity of chemotherapy on the A549 cells. |
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