Min Tang,Jun Bai,Chunyan Chen,Yingxia Ning,Xiaochun Li,Hanzhen He. The inhibiting effects of Laggera alata flavone on human ovarian cancer HO-8910 cells proliferation and its mechanism in vitro. Oncol Transl Med, 2014, 13: 427-431.
The inhibiting effects of Laggera alata flavone on human ovarian cancer HO-8910 cells proliferation and its mechanism in vitro
Received:June 30, 2014  Revised:August 29, 2014
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KeyWord:ovarian cancer; Laggera alata flavonen (LAF); apoptosis
Author NameAffiliationE-mail
Min Tang Hunan Provice Mawangdui Hospital, Changsha 410016, China shushuanlao@163.com 
Jun Bai Hangzhou Red Cross Hospital, Hangzhou 310003, China  
Chunyan Chen Hunan Provice Mawangdui Hospital, Changsha 410016, China  
Yingxia Ning The First Affiliated Hospital of Guangzhou Medical University, Guangzhou shushuanlao@163.com 
Xiaochun Li Department of Obstetrics and Gynecology, Longgang District Maternal and Child Healthcare Hospital of Shenzhen,  
Hanzhen He Department of Information, Longgang District Maternal and Child Healthcare Hospital of Shenzhen, Shenzhen  
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Abstract:
      Objective: The purpose of this study was to investigate the effect of Laggera alata flavonen (LAF) on the inhibiting effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods: Human ovarian cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide (PI) staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results: LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group (P < 0.05), and the IC50 was 4.28 μg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable difference compared with NS group (P < 0.05). Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion: LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro.
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