| Xueshuang Mei,Hongyi Hu,Guohui Nie. MiR-210 regulates cell cycle in nasopharyngealcarcinoma cell line (CNE-1) under hypoxic condition by reducing the expression of cyclin D1. Oncol Transl Med, 2014, 13: 323-327. |
| MiR-210 regulates cell cycle in nasopharyngealcarcinoma cell line (CNE-1) under hypoxic condition by reducing the expression of cyclin D1 |
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| KeyWord:nasopharyngeal carcinoma; miR-210; cyclin D1; cell cycle |
| Author Name | Affiliation | | Xueshuang Mei | Department of ENT, Peking University Shenzhen Hospital, Shenzhen 518036, China | | Hongyi Hu | Department of ENT, Peking University Shenzhen Hospital, Shenzhen 518037, China | | Guohui Nie | Department of ENT, Peking University Shenzhen Hospital, Shenzhen 518038, China |
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| Abstract: |
| Objective: The aim of our study was to determine the underlying mechanism of miR-210 on regulation of the cell cycle in nasopharyngeal carcinoma cell line CNE-1, particularly through regulation of cyclin D1, under hypoxic conditions. Methods: The CNE-1 cell line was induced with hypoxia, and the expression levels of endogenic miR-210 and cyclin D1 were detected by real-time PCR and Western blotting. Next, the luciferase assay was used to confirm that cyclin D1 is a target gene for miR-210. Cell cycle and cell proliferation were detected in CNE-1 cells that were cultured under hypoxic conditions with either overexpression or knockout of miR-210 using flow cytometry and MTT assay, respectively. Results: Hypoxia induced the expression of miR-210, resulting in reduced mRNA and protein levels of cyclin D1 and repression of cyclin D1 in CNE-1 cells. Further analysis indicated that miR-210 directly binded to the 3’UTR of the cyclin D1 gene, thus regulated the expression of cyclin D1. The flow cytometry assay showed that, under hypoxic conditions, miR-210 blocked CNE-1 cells in the G1 phase, and miR-210 also inhibited the proliferation of CNE-1 cells. Conclusion: Under hypoxic conditions, miR-210 directly reduced the expression of cyclin D1, leading to CNE-1 cells blocked in G1 phase. |
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