| Qian Wang,Jie Liu,Zewu An. Expression of CK19 mRNA and MUC-1 mRNA in the peripheral blood of patients with colorectal cancer and their clinical significances. Oncol Transl Med, 2014, 13: 316-319. |
| Expression of CK19 mRNA and MUC-1 mRNA in the peripheral blood of patients with colorectal cancer and their clinical significances |
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| KeyWord:colorectal cancer (CRC); peripheral blood; CK19 mRNA; MUC-1 mRNA; nested reverse transcription-polymerase chainreaction (Nested RT-PCR) |
| Author Name | Affiliation | | Qian Wang | Department of General Surgery, The No. 252 Hospital of PLA, Baoding 071000, China | | Jie Liu | Department of Neurology, The First Central Hospital of Baoding, Baoding 071000, China | | Zewu An | Department of General Surgery, The No. 252 Hospital of PLA, Baoding 071000, China |
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| Abstract: |
| Objective: Using nested reverse transcription-polymerase chain reaction (Nested RT-PCR) to test the mRNA level in peripheral blood CK19 and MUC-1 in colorectal cancer patients and it’s clinical significance, to discuss the feasibility of colorectal carcinoma micro-metastasis detection of molecular markers. Methods: The expression level was detected by nested RT-PCR in 20 healthy people, 20 patients with colorectal adenoma and 90 cases of patients with colorectal cancer disease peripheral blood CK19 mRNA and MUC-1 mRNA. Results: The positive expression rate of CK19 mRNA and MUC-1 mRNA were: 58.89% (53/90) and 52.22% (47/90). No CK19 mRNA healthy people 20 cases in the control group in the peripheral blood, the expression of MUC-1 mRNA in 12 cases, the expression rate of 60% (12/20). In 20 cases of colorectal adenoma diseases have the expression of CK19 mRNA in 1 cases, the expression rate of 5% (1/20), the expression of MUC-1 mRNA in 10 cases, the expression rate of 50%. Patients with colorectal cancer CK19 mRNA, MUC-1 mRNA expression rate was significantly correlated with tumor staging, the degree of differentiation of the tumor cells and tumor metastasis (P < 0.05). Conclusion: Marker CK19 mRNA as the detection of micro-metastasis in peripheral blood of patients with colorectal cancer has good sensitivity and specificity, but CK19 mRNA, MUC-1 mRNA can be used to judge the prognosis of patients with colorectal cancer index. |
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