| Sunan Wang,Yingying Li,Zhengshun Xu,Wenzhao Zhao,Tian Yun,Wuling Zhu,Yangkun Wang. Study on the protein expression and amplification of HER2 gene in gastric cancer. Oncol Transl Med, 2014, 13: 267-272. |
| Study on the protein expression and amplification of HER2 gene in gastric cancer |
| |
| View Full Text View/Add Comment Download reader |
| KeyWord:stomach neoplasms; mixed tumor; human epidermal growth factor receptor 2 (HER2) gene; HER2 protein; fluorescence in situ hybridization (FISH); immunohistochemistry (IHC) |
| Author Name | Affiliation | | Sunan Wang | Institute of Computer Technology, School of Electronics & Communication Engineering, Shenzhen Polytechnic,
Shenzhen 518055, China | | Yingying Li | Institute of Computer Technology, School of Electronics & Communication Engineering, Shenzhen Polytechnic,
Shenzhen 518055, China | | Zhengshun Xu | Department of Pathology, School of Medicine, Henan University of Science and Technology, Luoyang 471000,
China | | Wenzhao Zhao | Gstrointestinal Surgery, The First Affiliated Hospital, School of Medicine, Henan University of Science and
Technology, Luoyang 471000, China | | Tian Yun | Department of Pathology, No. 150 Hospital of PLA, Luoyang 471031, China | | Wuling Zhu | Department of Pathology, Basic Medical College of Xinxiang Medical University, Xinxiang 453000, China | | Yangkun Wang | Department of Pathology, No. 150 Hospital of PLA, Luoyang 471031, China |
|
| Hits: 7163 |
| Download times: 0 |
| Abstract: |
| Objective: The aim of the study was to investigate the human epidermal growth factor receptor 2 (HER2) gene amplification and protein expression and interpretation points in the stomach mixed carcinomas. Methods: Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) technique were used to detect HER2 gene amplification and expression of HER2 protein in 442 cases of gastric mixed carcinoma. Results: The expression rate of HER2 protein was 41.2% (182/442): the HER2 protein expression IHC 3+ extensive type in 18 cases, partial type in 21 cases, focal type in 8 cases, accounting for 10.6% (47/442); the HER2 protein expression IHC 2+ extensive type in 23 cases, partial type in 28 cases, focal type in 11 cases, accounting for 14.0% (62/442); the HER2 protein expression IHC 1+ extensive type in 27 cases, partial type in 31 cases, focal type in 15 cases, accounting for 16.5% (73/442). HER2 gene amplification rate of 442 cases was 16.1% (71/442). In 182 cases of HER2 protein positive expression, the HER2 gene cluster amplification rate was 14.8% (27/182), large granular amplification rate 11.0% (20/182), punctate amplification rate 6.0% (11/182) and high polysomy 7.1% (13/182). In 71 cases of HER2 gene amplification, there was 42 cases of HER2 protein expression IHC 3+, 22 cases of HER2 protein expression IHC 2+, and 7 cases of IHC 1+. Conclusion: HER2 detection of gastric mixed carcinoma has great heterogeneity, HER2 protein positive expression is divided into extensive type, partial type and focal type, and HER2 gene positive amplification is divided into cluster amplification, large granular amplification, punctate amplification and high polysomy. These typing of HER2 protein expression and HER2 gene amplification provide reference index to quantify for targeted therapeutic effect of anticancer drugs. |
| Close |
|
|
|